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Advanced Techniques

Advanced Techniques

PGD or preimplantation genetic diagnosis is a latest technique, which marries the recent spectacular advances in molecular genetics and assisted reproductive technology (ART). Before the establishment of pregnancy, It enables the doctors to identify genetic diseases in the embryo prior to implantation.

It enables people with an inheritable condition in their family to avoid passing it on to their children. It involves testing the genes and/or chromosomes of embryos created through IVF.

In Vitro Fertilization (IVF), because it is being done outside the body, has the advantage to undergo a testing to identify genetic defects in embryos. Amniocentesis or Chorionic villus sampling done to diagnose genetic defects are carried out during pregnancy; in case of test resulting positive, a very difficult step like termination of pregnancy is the only option to go for.

But Preimplantation Genetic Diagnosis and Screening (PGD/PGS) has empowered the doctors to save the couple from getting into such problems.

Preimplantation genetic testing is a technique used to identify genetic defects in embryos before implanting it in the womb of the woman. It is usually performed when couples are seen to have genetic disorders or when there has been such case in the family history.


What is PGS- (pre-implantation genetic screening)?

(PGS) is a new technology that has become recently available in India. Poojan IVF center is the first private IVF center in the country to have its own in - house Genetics Lab - Poojan IVF, offering Preimplantation Genetic Screening (PGS) using the highly sophisticated and cutting edge technology of the next Generation Sequencing (NGS) platform, with the fastest turnaround time for results. NGS technology aims to improve pregnancy by deselecting the embryos that have been tested to have a normal number of chromosomes. Earlier in the absence of PGS, embryos were chosen based only on the virtual quality and morphology, which cannot distinguish chromosomally normal embryos from abnormal ones. In IVF cycles that incorporate PGS, embryos are assessed by their chromosome content and are more likely to result in a pregnancy that is carried to full term.

PGS (also called aneuploidy screening) involves checking the chromosomes of the embryos conceived by IVF/ICSI for common abnormalities. Chromosomal abnormalities are a major cause of the failure of embryos to implant and of miscarriages/abortions. They can also cause conditions like Down’s syndrome.

PGS is recommended for women above 37 years of age, couples with a family history of chromosome problems, resurrect miscarriages, repeated IVF failures and severe male factor infertility. The procedure also goes a long way in reducing the likelihood of miscarriage, decreases the risk of abnormal pregnancy, reduces the time to achieve a pregnancy and additional costs inferred with multiple IVF cycles and increases the likelihood of pregnancy with single embryo transfers (eSET), thereby decreasing the multiple pregnancy rates like twins and triplets that are often seen in IVF cycles. In this procedure all 24 chromosomes (22 autosomes plus X and Y) are examined to evaluate any gains or losses of chromosomes, also known as aneuploidy.


The incubators are the soul of every In Vitro Fertilization (IVF) laboratory since they take care of the embryos while they grow mimicking the conditions of a mother’s womb. Traditionally incubators are unable to display other variables apart from the culture conditions and the embryos inevitably have to be taken out for daily evaluation interrupting controlled culture conditions.

Time Lapse Embryo Imaging can be used safely and continuously to monitor embryos during the whole incubation period. The EmbryoScopeTM is a revolutionary IVF incubator with a built-in microscope, which combines time-lapse imaging of the embryos during the culture and the latest incubator technology. This allows embryo development to be analyzed on high resolution while maintaining the best conditions in this state-of-the-art incubator. The deep and precise information about the embryo developmental patterns derived from analysis of the time-lapse images is also used as part of the selection process of the very best embryo/s for transfer.


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